Figure 3.

Nuclear localization of PthA4^AT is needed to trigger host defence response in Citrus limon. Phenotypic response of Xanthomonas citri 306 ∆pthA4 transformed with the different constructs was evaluated in C. limon leaves inoculated by pressure infiltration or cotton swab. Photographs were taken 15 days post‐inoculation and the insets show amplification of symptoms. NEC, non‐eruptive canker; HR, hypersensitive response. ∆NLS^AT:PthA4^AT derivative, with an 83‐amino acid deletion in the C‐terminal region, eliminating the three conserved nuclear localization signals (NLS); mutNLS^AT:PthA4^AT derivative mutated in the three NLS; ∆NLS^AT‐SV40:∆NLS^AT derivative containing the NLS of SV40. Scale bar: 10 mm.