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. 2019 Sep 23;10(10):1450–1456. doi: 10.1021/acsmedchemlett.9b00273

Figure 3.

Figure 3

(A) Structure of the dual luciferase reporter plasmid containing several nonsense mutational diseases sequence. The sequence below each PTC containing plasmid means the corresponding wild-type plasmid. (B) In vitro readthrough efficiency against several nonsense mutation sequences relative to control (D-MEM) in COS-7 cells; compounds were evaluated at a concentration of 200 μM. Values are expressed as the mean ± SD (n = 3). **p < 0.01 and *p < 0.05. (C) PTC readthrough at endogenous p53 R213X in HDQ-P1 human breast carcinoma cells. HDQ-P1 cells were exposed to different concentrations of TCP-112, TCP-1109, or G418 for 72 h, and full-length p53 (FL-p53) and truncated p53 (TR-p53) were determined by automated capillary electrophoresis western analysis. Vinculin was used as a protein loading control. The results are displayed as pseudo blots. FL-p53 peak intensity (p53 readthrough) is displayed under each lane, normalized to vinculin.