Fig. 3.
CDK11p110 localizes to the nucleus and exhibits high expression in tumor cells. a Schematic showing CDK11 chromosome exon locations, specifically the translated site that codes two protein isoforms. The CDK11p58 isoform is generated by an internal ribosome entry site sequence (IRES) in the same mRNA encoding the CDK11p110 isoform. b GFP-CDK11p110 and GFP-CDK11p58 transient transfection revealed that CDK11p110 is localized only in the nucleus, while CDK11p58 is expressed in the cytoplasm in three different cell lines (original magnification, 200×). c U-2OS-GFP-CDK11p110 and U-2OS- GFP-CDK11p58 selective stable cell lines show that CDK11p110 is localized only in the nucleus, while CDK11p58 is mainly expressed in the cytoplasm (original magnification, 200×). d CDK11p110 is highly expressed in U-2OS and KHOS cell lines; on the contrary, CDK11p58 is almost undetectable in these cell lines