Fig. 6.

CBFβ contributes to the proliferation and metastatic behavior of osteosarcoma cells. a Confirmation of knockdown of CBFβ protein expression by Western blotting. CBFβ expressions were abrogated by CBFβ siRNA in a dose-dependent manner. b U-2OS cells were transfected with increasing concentrations of CBFβ siRNA, and cell proliferation after transfection was determined by MTT assay (n = 3). c U-2OS cells were transfected with CBFβ siRNA or non-specific siRNA, and cell proliferation after transfection was determined by MTT assay. d KHOS cells were transfected with increasing concentrations of CBFβ siRNA, and cell proliferation after transfection was determined by MTT assay. e KHOS cells were transfected with CBFβ siRNA or non-specific siRNA, and cell proliferation after transfection was determined by MTT assay. f Migration distance of U-2OS for each time point and condition. g Migration distance of KHOS for each time point and condition. h The average number of invasive U-2OS cells among those transfected with CBFβ siRNA or non-specific siRNA in 24 h. i The average number of invasive KHOS cells among those transfected with CBFβ siRNA or non-specific siRNA in 24 h. *P < 0.05; **P < 0.01 (comparison of transfected cells with control cells using Student’s t-test)