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. 2019 Oct 15;12:246. doi: 10.1186/s13068-019-1585-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Design and optimization strategies for transcriptional biosensors. The performance of transcriptional biosensors can be altered by fine-tuning the expression levels of the allosteric transcription factor (1), engineering the cognate promoter and operator sequences (2), and/or ribosome binding site (3). The biosensor substrate recognition scope can be altered by engineering the specificity of the transcription factor (4)