Fig. 5.

Venn and Sankey diagrams showing between-line, within-line DEGs (|log₂(fold change)| ≥ log₂(1.2) and q < 0.05) at different time points. a Venn diagram showing 334 DEGs between the two lines at each time point. b and c Venn diagrams showing DEGs between time points post LPS treatment and baseline in the high-RFI (b) and low-RFI (c) animals. d-f Venn diagrams showing the relationship of within-line DEGs at 2, 6, and 24 hpi relative to baseline, respectively, between low-RFI and high-RFI lines. HRFI, high-RFI line; LRFI, low-RFI line. g Sankey diagram showing dynamic differential expression of genes at 2, 6 and 24 hpi compared to baseline for both lines. Flow strips of up-regulated, non-significantly differentially expressed, and down-regulated genes at 2 hpi in the high-RFI animals compared to baseline are colored in red, gray and green, respectively. The number of genes in each differential expression categories at each time point for each line is shown. The heights of the strips and blocks are proportional to the gene counts. Labels on the horizontal axis are designated similarly. For example, H2/H0 means gene expression at 2 hpi compared to baseline for the high-RFI animals