Table 1.

Demographics of participants for the pooled human sperm WGBS (WGBS-Pool) library.

Characteristics	$n$, $\text{mean}\pm \text{SD}$, and/or range
Age
Mean	$41.4\pm 9.1\mathrm{y}$
Range	27–61 y
Imprinted gene methylation
H19 (mean, range)	$97.1\pm 0.8\%$, $94.5–97.9\%$
MEST (mean, range)	$4.2\pm 1.0\%$, $2.7–6.2\%$
Fertility statusa
Fertile	21
Infertile	6
Unknown	3
Sperm counts (million/mL)
Mean	$100.8\pm 95.6$ ($n=29$)b
Range	3.8–497.7
Percentage DFI (%)
Mean	$15.0\pm 7.1$ ($n=26$)b
Range	6.05–30.9
Smoking status (n)
Smokers	6
Nonsmokers	23
Unknown	1
MTHFR genotype (n)
MTHFR CC	13
MTHFR CT	2
MTHFR TT	15
High-dose folic acid use [$5\mathrm{mg}/\mathrm{d}$ (n)]
Yes	6
No	24

Note: DFI, DNA fragmentation index; MTHFR, methylenetetrahydrofolate reductase; SD, standard deviation; WGBS, whole genome bisulfite sequencing.

^aA fertile status was determined if participants were normospermic and presented to the clinic due to known female factor infertility (see “Methods” section).

^bSperm counts and %DFI were not measured in all individuals due to limited amount of sample available upon collection.