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. 2019 Jun 5;127(6):067003. doi: 10.1289/EHP4583

Figure 1.

Figure 1A is a schematic diagram showing the measurement of Synchronous Ca2+ Oscillations development changes. Figure 1B is a graph plotting primary cortical neuronal network at 2, 4, 6, 8, 10, and 12 DIVs (right y-axis) across time at 0, 200, 400, and 600 seconds (x-axis). Figure 1C is a graph plotting SCO frequency at stages 0, 1, 2, 3, 4, and 5 (left y-axis) and SCO amplitude at stages 0.0, 0.5, 1.0, and 1.5 (right y-axis) across DIVs 2, 4, 6, 8, 10, and 12 (x-axis).

Developmental patterns of SCOs in primary cortical neurons. (A) Schematic diagram outlining the measurement of SCO development changes in cultured cortical neuronal networks. (B) Representative traces of SCOs recorded from primary cortical neuronal network at 2, 4, 6, 8, 10, and 12 DIVs. (C) Quantification of the SCO frequency and amplitude at different developmental stages. The mean value of each plate was used as analysis unit. Each data point represents the mean±standard error of the mean  (SEM) from three independent cultures. One-way analysis of variance (ANOVA) followed by post hoc Bonferroni comparison was used to compare the statistical significance of SCO frequency and amplitude between different DIVs. Note: DIV, days in vitro; SCO, synchronous Ca2+ oscillations. *p<0.05; **p<0.01 vs. 2 DIV; ##p<0.01 vs. 4 DIV; $$p<0.01 vs. 6 DIV; &p<0.05 vs. 8 DIV.