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. 2019 Jun 5;127(6):067003. doi: 10.1289/EHP4583

Figure 2.

Figure 2A is a graphical paradigm showing measurement of Electrical Spike Activity development changes. Figure 2B shows four representative raster plots plotting DIVs 5, 8, 11, and 14 (y-axis), across time 100 seconds (x-axis). Figure 2C, 2D, 2E, and 2F are graphs plotting Number of active electrodes per well (ranging between 0 and 80); spike frequency per second (ranging between 0 and 400); burst frequency per second (ranging between 0 and 50; and network burst frequency per second (ranging between 0.0 and 0.4) (y-axis), respectively, across DIVs (ranging between 0 and 15) (x-axis), representing data as standard error of mean.

Developmental patterns of ESA in primary cortical neurons. (A) Graphical paradigm outlining the measurement of ESA development changes in cultured cortical neuronal networks. (B) Representative raster plots of ESA in the same neuronal cultures at 5, 8, 11, and 14 DIVs. (C–F) Quantification of the number of (C) active electrodes, (D) spike frequency, (E) burst frequency, and (F) network burst frequency in neuronal cultures at different DIVs. The mean value of each plate was used as the analysis unit. Each data point shown in (B–E) represents the mean±standard error of the mean  (SEM) from three independent cultures. Repeated measurements one-way analysis of variance (ANOVA) analysis was used to analyze the number of active electrodes, spike frequency, burst frequency, and burst frequency during in vitro development. No statistical significance was observed between two different DIVs when using post hoc Bonferroni comparison. Note: DIV, days in vitro; ESA, electrical spike activity.