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. 2019 Jun 5;127(6):067003. doi: 10.1289/EHP4583

Figure 6.

Figure 6A is a graphical paradigm showing subchronic exposure of deltamethrin. Figure 6B are representative images of neurons with V e h exposure and deltamethrin exposure. Figure 6C is a bar graph plotting length of axon at 100, 150, 200, and 20 micrometers (y-axis) across Veh deltamethrin exposure at 3, 10, 30, 100, 300, and 1000 nanomolars (x-axis). Figure 6D are representative images of neuronal morphology with immunostains of MAP-2B at 7 DIV. Figure 6E is a graph plotting number of intersections at 0, 2, 4, 6, 8, and 10 (y-axis) across radius from soma 10, 25, 40, 55, 70, 85, and 100 pixels (x-axis), representing data as standard error of mean. Figure 6F are two graphs plotting number of intersections for radius 30 and radius 60 on left y-axis and right y-axis, respectively, across V e h deltamethrin exposure at 10, 30, 100, 300, and 1000 nanomolars (x-axis), representing data as standard error of mean.

Subchronic effect of DM exposure on axonal and dendritic outgrowth. (A) Graphical paradigm outlining subchronic exposure of DM for axonal and dendritic outgrowth. (B) Representative images of neurons exposed to Veh (0.1% DMSO) and different concentrations of DM commencing 3 h after plating and immunostained with MAP-2B antibody at 2 DIV. (C) Quantification of the axon length in the absence and presence of DM exposure. (D) Image J–processed representative images of neuronal morphology immunostained with MAP-2B at 7 DIV. Neurons were continuously exposed to Veh (0.1% DMSO) or different concentrations of DM starting from 3 h after plating. (E) Quantification of numbers of intersections at different radius in the absence and presence of DM exposure. The number of intersections was plotted with different radius (pixels). (F) Quantification the dendritic complexity after DM exposure. The radii of 30 and 60 pixels were chosen to quantify the number of intersections. The total number of neurons tested was inserted in the bar graph, and axonal length and number of intersections of each neuron was used as analysis unit. Each data point represents the mean±standard error of the mean  (SEM) from three independent cultures. One-way analysis of variance (ANOVA) followed by post hoc Bonferroni comparison was used to compare the statistical significance between Veh and DM exposed groups. Arrowheads indicate the radii of 30 and 60 pixels, respectively. Note: DIV, days in vitro; DM, deltamethrin; DMSO, dimethylsulfoxide; Veh, vehicle. *p<0.05; **p<0.01 vs. Veh. Bar=50μm.