Figure 6.
Subchronic effect of DM exposure on axonal and dendritic outgrowth. (A) Graphical paradigm outlining subchronic exposure of DM for axonal and dendritic outgrowth. (B) Representative images of neurons exposed to Veh (0.1% DMSO) and different concentrations of DM commencing 3 h after plating and immunostained with MAP-2B antibody at 2 DIV. (C) Quantification of the axon length in the absence and presence of DM exposure. (D) Image J–processed representative images of neuronal morphology immunostained with MAP-2B at 7 DIV. Neurons were continuously exposed to Veh (0.1% DMSO) or different concentrations of DM starting from 3 h after plating. (E) Quantification of numbers of intersections at different radius in the absence and presence of DM exposure. The number of intersections was plotted with different radius (pixels). (F) Quantification the dendritic complexity after DM exposure. The radii of 30 and 60 pixels were chosen to quantify the number of intersections. The total number of neurons tested was inserted in the bar graph, and axonal length and number of intersections of each neuron was used as analysis unit. Each data point represents the from three independent cultures. One-way analysis of variance (ANOVA) followed by post hoc Bonferroni comparison was used to compare the statistical significance between Veh and DM exposed groups. Arrowheads indicate the radii of 30 and 60 pixels, respectively. Note: DIV, days in vitro; DM, deltamethrin; DMSO, dimethylsulfoxide; Veh, vehicle. *; ** vs. Veh. .