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. 2019 Jun 5;127(6):067003. doi: 10.1289/EHP4583

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EHP is an open-access journal published with support from the National Institute of Environmental Health Sciences, National Institutes of Health. All content is public domain unless otherwise noted.

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Figures 8A and 8C show representative traces of SCOs in cortical neuronal cultures from WT mice and mice with R yR1 super T4826I virgule T4826I mutation and representative SCO traces in the absence and presence of deltamethrin in WT and R yR1 super T4826I virgule T4826I neurons, respectively. Figure 8B is a bar graph plotting SCO frequency at 0, 1, 2, and 3 and SCO amplitude at 0.0, 0.2, 0.4, 0.6, 0.8, and 1.0 on left y-axis and right y-axis, respectively, across deltamethrin at 100 nanomolars (x-axis) for WT and R yR1 super T4826I virgule T4826I neurons, representing data as standard error of mean. Figure 8D is a bar graph plotting SCO frequency at inhibition of 20, 40, 60, 80, and 100 percentage and SCO amplitude at inhibition of 20, 40, 60, 80, and 100 percentage on left-axis and y-axis, respectively, across deltamethrin at 100 nanomolars (x-axis) for WT and R yR1 super T4826I virgule T4826I neurons, representing data as standard error of mean. — Impacts of $RyR 1^{T 4826 I / T 4826 I}$ mutation on DM-induced SCO alternation. (A) Representative traces of SCOs in cortical neuronal cultures from WT mice (top trace) and mice carrying $RyR 1^{T 4826 I / T 4826 I}$ gain-of-function mutation (bottom trace) recorded at 7 DIV. (B) Quantification of the SCO frequency and amplitude in WT and $RyR 1^{T 4828 I / T 4826 I}$ neurons. (C) Representative SCO traces in the absence and presence of DM ( $100 nM$ ) in WT (upper two traces) and $RyR 1^{T 4828 I / T 4826 I}$ neurons (lower two traces). Arrowhead indicates the addition of Veh (0.1% DMSO) or DM ( $100 nM$ ). The data from dashed line window were used to analyze the inhibitions. (D) Quantification of DM inhibitions on the SCO frequency and amplitude in WT and $RyR 1^{T 4828 I / T 4826 I}$ neurons. DM-induced response (% inhibition) was analyzed by normalizing to respective vehicle control (0.1% DMSO) of each genotype. The mean values of SCO frequency and amplitude of each plate were used as analysis unit. Each data points represents the $mean \pm standard error of the mean (SEM)$ from four independent sister cultures. A t-test was used to compare the statistical significance of SCO frequency and amplitude between WT and $RyR 1^{T 4826 I / T 4826 I}$ neurons as well as the SCO response of DM between two genotypes. Note: DM, deltamethrin; DMSO, dimethylsulfoxide; RyRs, ryanodine receptors; SCO, synchronous ${Ca}^{2 +}$ oscillations; Veh, vehicle; WT, wild type. * $p < 0.05$ ; ** $p < 0.01$ , WT neurons vs. $RyR 1^{T 4828 I / T 4826 I}$ neurons.