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. 2019 Jun 5;127(6):067003. doi: 10.1289/EHP4583

Figure 8.

Figures 8A and 8C show representative traces of SCOs in cortical neuronal cultures from WT mice and mice with R yR1 super T4826I virgule T4826I mutation and representative SCO traces in the absence and presence of deltamethrin in WT and R yR1 super T4826I virgule T4826I neurons, respectively. Figure 8B is a bar graph plotting SCO frequency at 0, 1, 2, and 3 and SCO amplitude at 0.0, 0.2, 0.4, 0.6, 0.8, and 1.0 on left y-axis and right y-axis, respectively, across deltamethrin at 100 nanomolars (x-axis) for WT and R yR1 super T4826I virgule T4826I neurons, representing data as standard error of mean. Figure 8D is a bar graph plotting SCO frequency at inhibition of 20, 40, 60, 80, and 100 percentage and SCO amplitude at inhibition of 20, 40, 60, 80, and 100 percentage on left-axis and y-axis, respectively, across deltamethrin at 100 nanomolars (x-axis) for WT and R yR1 super T4826I virgule T4826I neurons, representing data as standard error of mean.

Impacts of RyR1T4826I/T4826I mutation on DM-induced SCO alternation. (A) Representative traces of SCOs in cortical neuronal cultures from WT mice (top trace) and mice carrying RyR1T4826I/T4826I gain-of-function mutation (bottom trace) recorded at 7 DIV. (B) Quantification of the SCO frequency and amplitude in WT and RyR1T4828I/T4826I neurons. (C) Representative SCO traces in the absence and presence of DM (100  nM) in WT (upper two traces) and RyR1T4828I/T4826I neurons (lower two traces). Arrowhead indicates the addition of Veh (0.1% DMSO) or DM (100  nM). The data from dashed line window were used to analyze the inhibitions. (D) Quantification of DM inhibitions on the SCO frequency and amplitude in WT and RyR1T4828I/T4826I neurons. DM-induced response (% inhibition) was analyzed by normalizing to respective vehicle control (0.1% DMSO) of each genotype. The mean values of SCO frequency and amplitude of each plate were used as analysis unit. Each data points represents the mean±standard error of the mean  (SEM) from four independent sister cultures. A t-test was used to compare the statistical significance of SCO frequency and amplitude between WT and RyR1T4826I/T4826I neurons as well as the SCO response of DM between two genotypes. Note: DM, deltamethrin; DMSO, dimethylsulfoxide; RyRs, ryanodine receptors; SCO, synchronous Ca2+ oscillations; Veh, vehicle; WT, wild type. *p<0.05; **p<0.01, WT neurons vs. RyR1T4828I/T4826I neurons.