Figure 3.

SAG D1 has a diplonemid-like mitochondrial genome architecture. (a) Eight contigs from SAG D1 contained modules encoding fragments of mitochondrial proteins. Verified mitochondrial contigs were extracted from assembled SAG D1 and analysed for the presence of proteins normally encoded by euglenozoan mitochondrial genomes. The bold red line in the first fragment (ranging from nad5 to nad8) denotes a fragment that was also confirmed by PCR and Sanger sequencing. (b) SAG D1 Cox1 requires trans-splicing but not RNA editing. Several contigs contained Cox1 fragments which could be manually assembled into a contiguous transcript. The ‘?’ mark denotes a possible short gene module which a small extension of module 4 would render unnecessary. Note that Diplonema species have a 6× Uracil (UUUUUU) insertion between their modules 4 and 5.