Figure 1.

Phylogenetic position of the new choanoflagellate SAGs. Phylogenetic tree based on 117 sequences of the 18S rDNA gene, representing all that is known of the molecular diversity of choanoflagellates and unicellular holozoans, including environmental lineages. The phylogenetic analysis was inferred by maximum likelihood under the GTR+ Γ with IQ-TREE. Clades marked by a bullet (•) present high statistical split support, with values greater than 80% of SH-aLRT (bootstraps of single branch test) and greater than 95% of ultrafast bootstrap. Both indexes were computed with IQ-TREE. The remaining split supports obtained can be found at Figshare (https://doi.org/10.6084/m9.figshare.7819571.v1) in the tree file. The order and class names given are based on [30,41,42]. Choanoflagellates with transcriptomic data available are depicted with a red asterisk, and those with genomic data available are depicted with a blue hash. Choanoflagellates' craspedidan clades were named according to our phylogenomic analysis (figure 3). Clade 3 nomenclature and nomenclature within Acanthoecida are the same as in [27]. The Acanthoecida picture was taken from [28] and Craspedida pictures were taken in Nicole's King laboratory.