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. 2019 Aug 19;8(13):5903–5915. doi: 10.1002/cam4.2463

Figure 4.

Figure 4

GEM did not affect HK2 mRNA and protein level. A, Real‐time PCR analysis of HK2 mRNA expression after GEM treatment at the indicated concentration and time in SW1990 cells. B, Real‐time PCR analysis of HK2 mRNA expression after GEM treatment at the indicated concentration and time in MIAPaCa‐2 cells. C, Western blot analysis of HK2 monomer levels after GEM treatment at the indicated concentration and time in the whole cell lysis of SW1990 cells. D, Western blot analysis of HK2 monomer level after GEM treatment at the indicated concentration and time in the whole cell lysis of MIAPaCa‐2 cells. The results represented three independent sets of experiments. Abbreviations: GEM, gemcitabine; HK2, hexokinase 2