Fig. 5.

Evidence that peroxynitrite-induced mitochondrial dysfunction mediates oxidative stress-induced apoptosis.A, PC6-V and PC12-MnSOD cells were exposed to vehicle, 100 μm FeSO₄, 50 μmAβ, or 100 μm SNP for 12 hr, and levels of JC-1 fluorescence were quantified. Values are the mean ± SEM of determinations made in six separate cultures; *p < 0.01 compared with the value for vehicle-treated PC6-V cells and with each value in PC6-MnSOD cells. B, Cultures were pretreated for 2 hr with vehicle or cyclosporin A and were then exposed for 20 hr to 100 μm FeSO₄, 50 μm Aβ25–35, or 100 μm SNP. Values are the mean ± SEM of determinations made in six cultures; *p < 0.01 compared with the control value; **p < 0.01 compared with the corresponding vehicle value.