Fig. 6.

Characterization of MnSOD expression in brain tissue of MnSOD transgenic mice. A, Northern blot analysis of MnSOD mRNA in brain tissue from Tg and wild-type (NTg) mice is shown. Poly(A+)RNA from the brains of Tg and NTg mice was separated on a 1.1% formaldehyde–agarose gel and, after transfer to nylon membrane, probed with a ³²P-labeled human MnSOD cDNA.B, Relative levels of MnSOD immunoreactivity in cerebral cortex were quantified in brain tissue sections (see Materials and Methods). Values are the mean ± SEM (n = 4 mice/group); *p < 0.01 compared with the corresponding WT value.