Fig. 7.

Cultured hippocampal neurons were fixed and stained for NR1 and neurofilament (NF) after 10 d in culture (see Materials and Methods). The dendritic processes of two neurons are shown to be diffusely stained for both NR1 (A) and NF (B), whereas an overlying axon (arrowhead inA, B) is stained only for NF. All NR1 staining was peptide blockable and was absent from the glial mat underlying these neurons. In C–E a higher-power view of another axon, terminating in a growth cone, is displayed. NR1 staining is restricted to the growth cone, whereas NF stain extends throughout the axon as well as the growth cone.