Fig. 1.

Western blot detection of the sst₁receptor in rat hypothalamus. Membranes prepared from hypothalamic tissue (25 μg/lane) were separated by SDS-PAGE, transferred to nitrocellulose, and reacted with anti-sst₁ antiserum diluted 1:1000. A band of an apparent molecular weight of ∼80,000 reacted specifically with the anti-sst₁ antiserum (lane B). This band was not detected using antiserum preabsorbed with the sst₁ antigen (lane A). A weak band placed immediately below the 80,000 molecular weight band was detected also with the preabsorbed antiserum and thus probably does not represent the sst₁ receptor. Molecular weights are indicated in kilodaltons.