Fig. 10.

Isolation of Ca²⁺currents. All recordings, except for C, were made with Cs⁺ internal solution. A, A sag in the inward current was evident in hair cells in the semi-intact preparation but not in enzymatically dissociated hair cells (B). C, NMG internal solution blocked the sag. The corresponding voltage steps (corrected for series resistance errors) are indicated by each trace (A–C). D, Peak Ca²⁺ current I–Vrelationships from enzymatically dissociated hair cells (□,n = 12) and hair cells in the semi-intact preparation (•, n = 10). E, Ca²⁺ current activation kinetics (τ_m, see text for definition) versus membrane potential (symbols are the same as in D: □,n = 11; •, n = 7). All recordings were made in the whole-cell configuration with 10 mm extracellular Cs⁺ to eliminate the activation of a K_IR current during the leak pulses. Values in D and E are mean ± SEM.