Fig. 4.

Effects of kinase inhibitors and an inhibitor of protein synthesis on the FGF-induced upregulation of Kv3.1 mRNA in P8 cerebellum. Slices were treated with 100 ng/ml bFGF in the presence of 50 μm AFC (an inhibitor of ras), 10 μm KN-62 (CaM/kinase inhibitor), 10 μmKT5720 (PKA inhibitor), 2.5 μm BIM I (PKC inhibitor), and 5 μg/ml cycloheximide in ACSF for 6 hr. The change in Kv3.1a mRNA levels induced by bFGF is significantly different from the changes produced by bFGF in the presence of KT5720, KN-62, AFC, BIM, and cycloheximide, with p < 0.05, 0.05, 0.005, 0.005, and 0.001, respectively, using two-tailed Student’s ttests (n = 3). The FGF-induced change in Kv3.1b mRNA levels is significantly different from that produced by FGF plus cycloheximide; p < 0.05 (n = 3).