Table 2.

The changes in the Kv3.1a and Kv3.1b mRNA levels induced by neurotrophins and depolarization during development

Treatment	Changes in the levels of the Kv3.1a and Kv3.1b mRNA (%)
	P3		P8		P15
	Kv3.1b	Kv3.1a	Kv3.1b	Kv3.1a	Kv3.1b	Kv3.1a
BDNF	61.5  ± 16.6*	63.7  ± 38.3	14.4  ± 11.3	3.5  ± 23.5	7.9  ± 19.1	9.7  ± 42.9
NT-3	25.8  ± 21.3	43.6  ± 13.4**	34.5  ± 8.3	14.5  ± 23.2	−8.5  ± 4.4	0.9  ± 23.1
High-K	14.1  ± 13.9	40.6  ± 21.2	8.3  ± 6.8	7.0  ± 17.2	−7.5  ± 13.4	−5.9  ± 4.1

Cerebellar slices were incubated with BDNF (100 ng/ml), NT-3 (100 ng/ml) in ACSF, or ACFS containing 50 mm KCl, at room temperature for 6 hr. The mRNA levels of Kv3.1a, Kv3.1b and GAPDH were measured by the RNase protection assay. The values (mean ± SEM) of P3, P8, and P15 are averages of two to five RNA preparations. The values are significantly different from 0 with p < 0.05 (n = 4) (*) and 0.05 (n = 5) (**), determined by two-tailed Student’s t test.