Fig. 3.

Localization of NMDAR1 isoforms in striatal neuronal nitric oxide synthase (nNOS)-containing neurons. The panels on the left illustrate immunohistochemical staining for the common region of NMDAR1 (A), the N1 segment (C), and the C1 segment (E). The panels on the right,B, D, and F, illustrate staining of the same sections for nNOS. Staining for NMDAR1 is found in nearly all striatal neurons, including those containing nNOS (A, B). The antibody to the N1 segment stains produces staining of the striatal neuropil as well as low-level staining of the cytoplasm of neurons; the intensity of this staining in nNOS-positive and nNOS-negative cells is similar (C,D). The antibody to the C1 segment produces robust cytoplasmic staining of most striatal neurons, but it does not stain those that contain nNOS (E, F). Scale bar, 20 μm.