Fig. 3.

Enhancement of FN-based retinal neurite outgrowth by F11. Retinal cells of embryonic day 6 were cultured for 20 hr on FN alone (left column) or on a mixture of FN and F11 (right column). Cells and neurites were identified by immunofluorescence analysis using NCAM-specific antibodies, and cellular nuclei were identified by bisbenzimide staining. Thefirst row shows immunofluorescence micrographs, and thesecond through fourth rows show objects that have been processed by the image analysis procedure outlined in detail in Figure 1. In the second row cells and neurites are depicted; in the third row, the cellular nuclei; and in the fourth row, the identified neurites. Scale bar, 100 μm.