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. 1998 Mar 1;18(5):1743–1752. doi: 10.1523/JNEUROSCI.18-05-01743.1998

Fig. 2.

Fig. 2.

Change in specific activity of mouse brain APP with time. Mice were treated as reported in the legend to Figure 1 and Materials and Methods. A, Representative phosphorimage showing radiolabeled immature (i) and fully glycosylated (m) APP695. B, C, Time-dependent change in specific activity of immature (B) and fully glycosylated APP695 (C). n = 3 at each time point. D, Representative Western blot showing relatively constant absolute levels of APP, although specific activity was changing. E, Immunoblot confirming the predominant forms of APP synthesized by gene-targeted mouse. The most prominent band in the gene-targeted mouse brain sample (lane 2) co-electrophoreses with immature human APP695 from transgenic rat brain (lane 1) and lowest APP form isolated from human cortex (lane 3). The top band in the full-length APP complex (lane 2) co-electrophoreses with fully glycosylated human APP 695 from transgenic rat (lane 1).