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Western blot analysis of selected fractions from gel filtration chromatography of serum-free conditioned media from transgenic astrocytes expressing apoE3 (E3, line 37) or apoE4 (E4, line 22). Human apoE, as detected with rabbit anti-human apoE antisera, was present in fractions eluting in the size range of plasma HDL. The blot was developed with ECL. Arrows indicate positions of apoE (∼36 kDa) monomer (E3, E4), and (∼72 kDa) dimer (E3).
