Table 1.

Relative fluorescence of Ca Crimson at nerve terminals before and after stimulation of the motor nerve

Preparation	Temperature (°C)	Fstim/Frest
Wild-type control (4)	20	1.52  ± 0.23
	32	1.43  ± 0.20
csp mutant (6)	20	1.26  ± 0.20
	32	0.95  ± 0.07
	20	1.18  ± 0.11
shibire mutant (4)	20	1.51  ± 0.27
	32	0.99  ± 0.03
	20	1.21  ± 0.10

For each nerve terminal we obtained a meanF_stim/F_rest from individual boutons (≥10 boutons per terminal). For nterminals (in separate preparations) a population mean (±SD) was computed and is presented here asF_stim/F_rest.