Fig. 1.

Response in neurons and astrocytes to stimulation with 40 mm KCl. A, Time series of pseudocolor images of the [Ca²⁺]_ichanges occurring in Indo-1-loaded cells from CA1 hippocampal region of a young rat (at P6) after perfusion of the slice with 40 mmKCl. The sequence shows the [Ca²⁺]_iincrease in pyramidal neurons (closed arrows) occurring several seconds before that in astrocytes (open arrows). The R405:485 is displayed as a pseudocolor scale. Sampling rate, 2 sec; scale bar, 10 μm. B, Kinetics of the [Ca²⁺]_i changes in the neurons (continuous lines) and the astrocytes (dotted lines), indicated by the arrows inA, after K⁺ stimulation, as expressed by the ratio between Indo-1 emission wavelength at 405 and 485 nm.a–h, Images a–h inA.