Fig. 7.

Effects of GGF2 on adult OPCs. Purified P60 and P8 OPCs were cultured for 8 d at clonal density in serum-free medium containing PDGF, GGF2,IBMX (0.1 mm), and forskolin (5 μm), as indicated. A, The division rate of P8 and P60 was calculated from the clone size. B, The rate of oligodendrocyte generation by P60 OPCs was determined by the percentage of clones that primarily consisted of oligodendrocytes. GGF2 enhances the rate of proliferation of P60 OPCs and inhibits their differentiation into oligodendrocytes. All values are mean ± SEM.FORSK, Forskolin; IBMX, isobutylmethylxanthine.