Fig. 3.
Transcription analysis of the targeted IRBP gene.A, Northern blot analysis of the total RNA isolated fromIRBP+/− and IRBP−/− mice. Ten micrograms of total RNA were run on a formaldehyde–agarose gel and transferred to a Zeta Probe membrane. The top paneldepicts the structure of the wild-type and targeted IRBP alleles in each genotype. The blot was hybridized with the following probes in sequence: mouse IRBP cDNA, the pgk-neofragment, and a mouse β-actin gene. R, Retina;B, brain; K, kidney; L, liver; S, spleen; T, testis; ΔIRBP, the remaining segment of the IRBP gene.B, RPA of total retinal RNA from IRBP+/+ and IRBP−/− mice. One microgram of total RNA was hybridized with 32P-labeled RNA probes from the coding (sense) or noncoding (antisense) strand of the mouse IRBP cDNA.