Properties of cAMP-modulated outward currents in mushroom body neurons. Mushroom body neurons were identified under the fluorescence microscope as described in Materials and Methods. Currents shown in records labeled 3 were obtained by subtraction from control outward currents in records labeled 1 and outward currents recorded after exposure to X-cAMP or 100 μm 4-AP in records labeled 2.A, cAMP-modulated outward currents in mushroom body neuron. Top record, Maintained, cAMP-sensitive outward current component. Bottom record, Slowly inactivating, cAMP-sensitive outward current in mushroom body neuron.B, Blockade by 100 μm 4-AP of maintained and slowly inactivating outward currents in mushroom body neurons.Top record, 4-AP-sensitive, maintained outward current.Bottom record, 4-AP-sensitive, slowly inactivating outward current. Notice in record labeled 3 the presence of a fast-inactivating, 4-AP-sensitive outward current component.