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PSD-95 is present at excitatory synaptic sites, but not at inhibitory synapses. Hippocampal neurons fixed at 21 d in culture were immunostained for PSD-95 (A,C) and either GluR1 (B) or the GABAA receptor β2/3 subunit (D). PSD-95 clusters colocalized with GluR1-labeled excitatory synapses (indicated by arrows in A,B), but not with the GABAA receptor-labeled inhibitory synapses (arrows in C,D). Scale bar, 10 μm.
