Fig. 2.

Effects of nickel, thapsigargin, and t-ACPD on the number and size of neuronal domains. A, Average number of neuronal domains elicited by temperature drop under control conditions and in the presence of 2 mm nickel chloride (Ni) or 10 μm thapsigargin (thap). Blockade of voltage-gated calcium channels with 2 mm nickel had no effect on the number of domains. In contrast, depletion of intracellular calcium stores with 10 μm thapsigargin almost completely abolished neuronal domains (p < 0.01, Student’st test). B, Average size of neuronal domains elicited by temperature drop under control conditions and in the presence of 2 mm nickel (Ni) and elicited by bath application of t-ACPD (40–100 μm). Neuronal domains elicited by t-ACPD were smaller (p < 0.01) than were domains elicited by temperature drop. In all cases, the bath solution contained 2 μm TTX. Numbers abovebars indicate the number of slices (A) or number of neuronal domains (B). Asterisks indicate a significant difference (p ≤ 0.05; student’st test).