Fig. 3.
A, Activation of the metabotropic glutamate agonist t-ACPD elicits neuronal domains. Theleft and middle images were taken before and during the application of 40 μm t-ACPD. In theright image, changes in fura-2 emission are pseudocolor coded as ΔF/F (in percent) and overlaid onto the middle image. Each individual image is the average of 16 background-subtracted frames. Numerous neuronal domains are visible, as are individual cells, the [Ca2+]i of which increased by activation of metabotropic glutamate receptors. B, t-ACPD-elicited neuronal domains depend on functional gap junctions. In the presence of the gap junction blocker octanol (1 mm), application of 200 μm t-ACPD elicits only single-cell responses but no neuronal domains. The pial surface is to theupper right in A and up inB. A, Coronal slice of a P3 rat.B, Coronal slice of a P4 rat.