Fig. 2.

A, Quantification of motoneuron cell loss during pmn/pmn disease progression using the Fluorogold retrolabeling technique. Each point represents three animals. As early as day 33, the number of retrogradely labeled motoneurons is significantly lower in pmn as compared with controls (*p < 0.05). At 41 d of age the number of motoneurons is significantly lower than in controls and in 20-d-old pmn animals (**p < 0.01). B, The same sections were counterstained with cresyl violet and recounted. At day 41, the number of motoneurons is smaller than in controls or 20-d-old pmn mice (****p < 0.0001). Note the similarity in the pattern of motoneuron cell loss with the two techniques.