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Induction of neuronal nitric oxide synthase gene expression by trophic factor deprivation in cultured motor neurons. Shown is mRNA expression for neuronal NOS in cultured motor neurons by RT-PCR. Total RNA from 50,000 motor neurons was extracted 24 hr after plating. One-half of the RNA was incubated with reverse transcriptase (RT) before PCR (+RT), whereas the other one-half was amplified without RT (−RT). cDNA from neuronal NOS was used as the standard.
