Table 1.
l-Arginine reverses the protective effects of NOS inhibition
Treatment | Motor neuron survival* (%) | n |
---|---|---|
None | 35 ± 4 | 8 |
BDNF | 100 ± 4 | 8 |
l-NAME | 80 ± 6 | 4 |
l-NAME + l-arginine | 47 ± 3 | 4 |
l-NAME + d-arginine | 79 ± 4 | 4 |
d-NAME | 32 ± 6 | 4 |
*The percentage of motor neuron survival was determined 24 hr after plating, as indicated in Materials and Methods. The number of cells in BDNF-treated cultures at 24 hr was taken as 100%. Values are the mean ± SD of n determinations from at least two independent experiments. The concentrations were 100 pg/ml BDNF, 1 mml-NAME; 2 mml-arginine; 2 mmd-arginine; and 1 mmd-NAME.