Table 2.
Effects of NO on motor neuron survival
Treatment | Motor neuron survival* (%) | n |
---|---|---|
None | 35 ± 4 | 10 |
BDNF | 100 ± 4 | 10 |
BDNF + DETA-NONOate | 102 ± 7 | 6 |
DETA-NONOate | 31 ± 4 | 4 |
DETA | 35 ± 5 | 4 |
l-NAME + DETA | 79 ± 8 | 4 |
l-NAME + DETA-NONOate | 50 ± 6 | 6 |
l-NAME + DETA-NONOate + SOD | 27 ± 4 | 4 |
SOD | 37 ± 8 | 4 |
*The percentage of motor neuron survival was determined 24 hr after plating, as described in Materials and Methods. The number of cells in BDNF-treated cultures at 24 hr was taken as 100%. Values are mean ± SD of n determination from at least two independent experiments. The concentrations were 100 pg/ml BDNF, 20 μm DETA-NONOate; 20 μm DETA; 1 mml-NAME; and 1000 U/ml SOD. Similar results were obtained by using up to 25,000 U/ml SOD.