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. 1998 Feb 1;18(3):932–947. doi: 10.1523/JNEUROSCI.18-03-00932.1998

Fig. 5.

Fig. 5.

Nuclear DNA fragmentation demonstrated with BODIPY-dUTP simultaneously with CMTMR staining to estimate ΔΨM. A1, A2, Low-power epifluorescence micrographs of the same image fields of cells after 12 hr in M/O that were immunoreacted for tubulin (A1) and also reacted using the BODIPY-dUTP method for detecting nuclear DNA fragmentation (A2). Confocal laser images of identical fields for tissue dually reacted with an anti-histone antibody are shown inB1 and C1, and BODIPY-dUTP is shown inB2 and C2. D1–D3, E1–E3, Identical confocal image fields for serial image planes (separated by ∼1.0 μm) through a group of partially neuronally differentiated PC12 cells in M/O at 6 hr after washing. Cultures are stained for BODIPY-dUTP nuclear staining and CMTMR mitochondrial fluorescence, respectively.