Skip to main content
. 1998 Jun 1;18(11):4029–4041. doi: 10.1523/JNEUROSCI.18-11-04029.1998

Fig. 6.

Fig. 6.

Series of time-lapse confocal fluorescence images in the same optical midsection ∼100 μm from the cut end showing stages of injury-induced endocytosis beginning with an invagination of FM-labeled membrane and ending with a vesicle moving in the axoplasm toward the cut end of a transected MGA. A–F, The axon is at the top, the bath is at the bottom, and the cut end is toward the right. The plasmalemmal membranes of an intact MGA were pulse-labeled with FM 1-43. The MGA was transected and imaged for FM 1-43 fluorescence starting at 11 min after transection without changing the confocal plane or the position of the micrometer stage. A–F, Successive images were acquired at 18 sec intervals and taken from a larger set of images acquired every 6 sec. Asterisks mark the same vesicle in every frame. Note that vesicles are sometimes joined by a fluorescent line, presumably a tether of membranous material. Scale bar (inF), 10 μm.