Table 2.
The four functional CMTX mutations interact with Cx32wt and retain its same selective compatibility in heterotypic channel formation
| Oocyte injection (cell 1/cell 2) | Gj (μS) | Number of pairs |
|---|---|---|
| Antisense/antisense | 0.04 ± 0.03 | 5 |
| L56F/Cx32wt | 13.9 ± 3.3 | 9 |
| E102G/Cx32wt | 20.5 ± 6.4 | 11 |
| Del111–116/Cx32wt | 6.0 ± 1.9 | 10 |
| R220stop/Cx32wt | 6.4 ± 2.4 | 7 |
| L56F/Cx26 | 9.3 ± 2.5 | 6 |
| L56F/Cx46 | 9.6 ± 3.2 | 4 |
| L56F/Cx50 | 10.3 ± 3.2 | 5 |
| E102G/Cx26 | 5.0 ± 0.9 | 3 |
| E102G/Cx46 | 5.7 ± 1.4 | 4 |
| E102G/Cx50 | 3.6 ± 1.3 | 3 |
| Del111–116/Cx26 | 5.9 ± 0.8 | 10 |
| Del111–116/Cx46 | 4.8 ± 1.1 | 6 |
| Del111–116/Cx50 | 5.9 ± 1.0 | 5 |
| R220stop/Cx26 | 2.1 ± 0.7 | 5 |
| R220stop/Cx46 | 3.4 ± 0.9 | 7 |
| R220stop/Cx50 | 2.0 ± 0.6 | 5 |
| L56F/Cx40 | 0.06 ± 0.04 | 4 |
| E102G/Cx40 | 0.03 ± 0.01 | 6 |
| Del111–116/Cx40 | 0.01 ± 0.01 | 4 |
| R220stop/Cx40 | 0.01 ± 0.01 | 6 |
Antisense-treated cells were injected with the specified cRNAs, and measurements of steady-state junctional conductance (Gj) were performed 24–48 hrs after pairing, using a dual voltage-clamp procedure. Whereas the four functional CMTX mutations failed to interact with Cx40, homotypic Cx40/Cx40 pairs injected with the same RNA batch developed a robust junctional conductance (16.0 ± 0.2 μS; n = 3). Results are shown as the mean ± SEM of the number of pairs indicated.