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The PI3 kinase inhibitor wortmannin does not affect NAC-mediated survival. PC12 cells in serum-free medium were pretreated with 150 nm wortmannin for 30 min and then 60 mm NAC for 9 hr. Wortmannin was added every 2 hr to compensate for breakdown. Results are expressed relative to survival obtained in NGF-treated cells in the absence of wortmannin (n = 3).
