Table 3.

Effect of the PKC inhibitor peptide (19–31) on stimulus-induced MARCKS phosphorylation in the AL

Stimulation	Reduction of MARCKS phosphorylation by PKC inhibitor peptide
	− (Ca, DAG) (%)	+ (Ca, DAG) (%)
−	92  ± 4  (8)*	90  ± 3  (8)*
CS	91  ± 4  (4)*	91  ± 4  (4)*
US	89  ± 5  (4)*	90  ± 5  (4)*
3 hr after one trial	92  ± 5  (4)*	95  ± 4  (4)*
3 hr after three trials	95  ± 5  (4)*	91  ± 4  (4)*
18 hr after one trial	94  ± 5  (4)*	94  ± 5  (4)*
18 hr after three trials	92  ± 4  (4)*	93  ± 5  (4)*

Animals were dissected unstimulated (−), directly after stimulation (CS or US), or at 3 hr, respectively, 24 hr after training (one or three trials). MARCKS phosphorylation was measured in the presence or absence of (100 μm) PKC inhibitor peptide (19–31). Mean values ± SEM indicate the reduction of kinase activity by PKC inhibitor peptide. The number of independent measurements is shown in parentheses. Values that are significantly different from the corresponding values in the absence of the PKC inhibitor peptide are marked by an asterisk (p < 0.01; t test).