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. 1998 Jun 1;18(11):4177–4188. doi: 10.1523/JNEUROSCI.18-11-04177.1998

Fig. 2.

Fig. 2.

Generation of NR2D transgenic mice.A, Southern blot analysis of the six transgenic lines,1, 3, 7, 8,9, and 17. Successful transmission of the transgene was verified by the presence of a 6 kb BamHI fragment. The arrow indicates the position of the endogenous NR2D fragment. B, Northern blot analysis with a probe for NR2D transcript. The 6.5 kb transcript can be identified in lines 3, 9, and 17.Lane C, Transgene negative control. C,In situ hybridization of brain sections from transgenic and control mice with a transgene-specific probe (SV40polyadenylation signal) and a probe for NR2D. D, Immunoblot analysis of the postnatal change of NR2D proteins in control (C) and transgenic (T) mice hippocampi. Upregulation of NR2D protein level was observed between 2 and 8 weeks. E, Immunoprecipitation of NMDA receptor complex with an anti-NR1 antibody. The precipitates were analyzed by immunoblotting with anti-NR1, anti-NR2D, and anti-GluR1 antibodies. E, Extracts from line 9 transgenic forebrain; PT, immunoprecipitates from line 9 forebrain; PC, immunoprecipitates from control forebrain.