Table 1.

Base sequences of NR1 and NR2 oligonucleotide probes used for in situ hybridization, RT-PCR, and Southern blot analysis

Antisense oligonucleotide sequences for in situhybridization
NR1 pan	5′-GCC ATC TGC CAC CAG GTG CAC CTC GTA GGT GAA GTT CAT GGT-3′
NR1a	5′-CTG CAG CAC CTT CTC TGC CTT GGA CTC CCG TTC CTC-3′
NR1b	5′-GGG TCC GCG CTT GTT GTC ATA GGA CAG TTG GTC GAG GTT TTC-3′
NR1-1	5′-TCC ACC CCC GGT GCT CGT GTC TTT GGA GGA CCT ACG-3′
NR1-2	5′-GCG TCC ACC CCC GGT GCG CTG CAG GTT CTT CCT CCA-3′
NR1-3	5′-TAT CAG TGG GAT GGT ACT CTG CAG GTT CTT CCT CCA-3′
NR1-4	5′-TAT CAG TGG GAT GGT ACT GCT GCA GGT TCT TCC TCC-3′
NR2a	5′-AGA AGG CCC GTG GGA GCT TTC CCT TTG GCT AAG-3′
NR2b	5′-GGG CCT CCT GGC TCT CTG CCA TCG GCT AGG CAC CTG TTG TAA-3′
NR2c	5′-TGG TCC ACC TTT CTT GCC CTT GGT GAG GTT CTG GCT GTA GCT-3′
NR2d	5′-CAG CCC GGC AGG CAG TGG GGA ACC TCC TGG CAG AAG-3′
Primer sequences for RT-PCR
5′ NR1 exon 5	5′-GTG CCG CCC TAC TCC CAC CAG TCC-3′
3′ NR1 exon 5	5′-CAG AGC CGT CAC ATT CTT GGT TCC-3′
Base pair sequence of probes used for antisense targeting
Antisense NR1b	5′-GGG TCC GCG CTT GTT GTC-3′
Reversed antisense	5′-CCC AGG CGC GAA CAA CAG-3′