Left, Full-field view of a representative micrograph from the stack of images used for three-dimensional reconstructions. The shaft and two varicosities (var) of an axon are indicated. Right, Eight reconstructed axons from series LMTN, at the same scale as the micrograph. Some axons extend beyond the area of the micrograph because of the three-dimensional perspective. The axons travel in many different directions, rather than parallel to each other. Scale bar, 1.0 μm.