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. 1998 Oct 15;18(20):8126–8132. doi: 10.1523/JNEUROSCI.18-20-08126.1998

Fig. 2.

Fig. 2.

HPLC-ECD chromatogram of a protein digest from AD brain (SMTG region) chromatographed by using the same column as the sample shown in Figure 1, with the inclusion of LDS in the mobile phase. A, Original sample (full scale = 25 μA current) showing ECD response at 200 mV cell potential (channel 1). The peak marked in A (asterisk) coeluted near 3-NH2-Tyr but differed markedly with respect to oxidation potential (see Results). B, Original sample at 700 mV cell potential (channel 9) illustrating the peak assigned to 3-NO2-Tyr. C, D, Regions of the chromatogram shown in A and B, respectively, after treatment of the sample with hydrosulfite to partially reduce the 3-NO2-Tyr to 3-NH2-Tyr (labeled).