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. 1998 Nov 15;18(22):9312–9325. doi: 10.1523/JNEUROSCI.18-22-09312.1998

Fig. 1.

Fig. 1.

Characterization of Ntm-myc expression in transfected CHO cells. A, Immunofluorescence micrographs showing Ntm-myc expression on the surface of CHO-Ntm cells (panel 1) detected with an anti-myc antibody.Arrowheads indicate examples of the accumulation of Ntm at sites of cell–cell contact. Control cells transfected with the vector (panel 2) are not stained. Thebottom panels show the Hoechst staining of the corresponding fields. Scale bar, 50 μm. B, Lysates of surface-biotinylated CHO-Ntm and control cell monolayers were immunoprecipitated with the anti-myc antibody, electrophoresed, blotted, and probed with alkaline phosphatase-conjugated streptavidin. The prominent band migrating at 55–65 kDa, corresponding to the predicted size of Ntm, is present in immunoprecipitates of Ntm-myc cells but not control cells. Molecular weight markers are indicated atleft. C, Western blot, with the anti-myc antibody, of supernatants (S) and cell lysates (L) of CHO-Ntm cultures either treated (+) or mock-treated (−) with PI-PLC. A prominent band corresponding to Ntm-myc is present in the supernatant of PI-PLC-treated cultures but not in mock-treated cultures. Molecular weight markers are indicated atleft.