Table 1.

Summary of neurite outgrowth assays

	DRG	Hippocampal	SCG
Ntm-Fc (bound)
Ntm-Fc (100 μg/ml)	136.42  ± 23.27*	41.92  ± 3.94*	21.03  ± 3.07
Anti-Fc + Ntm-Fc	nd	56.76  ± 6.53*	nd
Ntm-Fc + PI-PLC	144.56  ± 23.4*	20.57  ± 0.38	nd
Laminin (10, 25, 10 μg/ml)	149.47  ± 0.89*	103.73  ± 5.82*	198.79  ± 34.49*
MUC18-Fc (100 μg/ml)	23.66  ± 1.63	19.87  ± 0.54	16.16  ± 2.37
Ntm-Fc (soluble)
Ntm-Fc (10 μg/ml)	109.33  ± 5.64*	nd	122.89  ± 29.24
MUC18-Fc (10 μg/ml)	72.18  ± 4.8	nd	191.88  ± 49.18
Transfected cells
CHO-Ntm	165.02  ± 14.38*	nd	149.90  ± 19.21*
CHO-Ntm + PI-PLC	129.52  ± 9.91	nd	218.02  ± 11.29
CHO-CMV	104.94  ± 4.25	nd	215.70  ± 12.26

This table summarizes the outgrowth of different primary neurons grown on Ntm-Fc bound to nitrocellulose, on extracellular matrix substrates with soluble Ntm-Fc added to the media, and on transfected CHO cells. Neurite outgrowth was quantified by image analysis, as described in Materials and Methods, and expressed as a mean neurite length ± SEM. At least three independent experiments were performed for each condition, except for the Anti-Fc + Ntm-Fc for which two experiments were performed. *Indicates a significant difference (p < 0.05) from control according to Student’s t test. nd, Not determined.