Fig. 8.

The effects of α-BgTx on the carbachol-induced enhancement of spontaneous PSCs. A, The carbachol-induced enhancement of spontaneous glutamatergic EPSCs is blocked by a 2 hr incubation with α-BgTx (0.1 μm) (top left), whereas in a separate neuron, α-BgTx did not block the carbachol-induced increase in GABAergic IPSCs (bottom left). Cumulative distributions of EPSC and IPSC intervals are shown on the right. B, In another neuron from the same slice, the enhancement of spontaneous glutamatergic EPSCs slightly recovered after 35 and 75 min of washout of α-BgTx. The neurons were clamped at −50 mV in ACSF with TTX (0.5 μm) and bicuculline (10 μm) and potassium gluconate in the recording electrode for glutamate and −70 mV in ACSF with TTX and potassium chloride in the recording electrode for GABA.Bars above records indicate 30 sec application of 100 μm carbachol.