Fig. 2.
Primary structure of yotiao. A, Aligned below the schematic of yotiao are the overlapping cDNA clones used to determine its full-length sequence. A1.7 is the clone isolated by yeast two-hybrid screen using as bait the NR1A C-terminal tail.Hatched regions within the schematic represent areas of high coiled coil probability as predicted by the Lupas algorithm (Lupas et al., 1991). The region of yotiao prepared as H6-tagged immunogen is shown aligned with full-length yotiao. B, The deduced primary structure of yotiao is shown in single-letter amino acid code.